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adipogenic differentiation medium  (Beijing Solarbio Science)


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    Beijing Solarbio Science adipogenic differentiation medium
    Adipogenic Differentiation Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adipogenic+differentiation+medium/Medium/pm41772692-75-38-48
    Average 99 stars, based on 10711 article reviews
    adipogenic differentiation medium - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Polydopamine-functionalized nanohydroxyapatite coated exosomes with enhanced cytocompatibility and osteogenesis for bone regeneration
    Article Snippet: Background: Mesenchymal stem cells-derived exosomes, crucial in regenerative medicine, have been explored for their potential for the functional modification of bone scaffolds.. Objective: To design a functionally modified biomimetic nanohydroxyapatite using exosomes and explore its effects on bone regeneration.. Methods: A biomimetic nanohydroxyapatite (named as tHA) was fabricated as previous methods using a polydopamine (pDA) structure as a template, and exosomes (Exo) derived from periodontal ligament stem cells (PDLSCs) were used to functionally modify the tHA scaffold material through pDA.

    Article Title: The enhanced osteogenic differentiation of human periodontal ligament stem cells and M2 polarization of macrophages may be mediated by EphB4/ephrinB2 signaling pathway: a study of their direct co-culture.
    Article Snippet: After air-drying at room temperature, cell clones were observed under the inverted microscope (NEXCOPE, USA), with a clone defined as containing ≥50 cells. .. Multilineage differentiation of hPDLSCs hPDLSCs in P3 were harvested and cultured in osteogenic differentiation medium (α-MEM supplemented with 10% FBS, 10 mmol/L β-glycerophosphate (Solarbio, China), 0.1 μmol/L dexamethasone (Solarbio, China) and 0.05 mmol/L vitamin C(Sigma; Merck, Germany) or adipogenic differentiation medium (α-MEM supplemented with10% FBS, 200 μmol/L indomethacin (Solarbio, China), 10 μmol/L dexamethasone (Sigma; Merck, Germany) and 0.5 mmol/L IBMX (Solarbio, China) for 21 days. .. Then, cells were fixed and stained with Alizarin Red S (Solarbio, China) for osteogenic differentiation or Oil Red O (Solarbio, China) for adipogenic differentiation respectively.

    Article Title: The enhanced osteogenic differentiation of human periodontal ligament stem cells and M2 polarization of macrophages may be mediated by EphB4/ephrinB2 signaling pathway: a study of their direct co-culture
    Article Snippet: After air-drying at room temperature, cell clones were observed under the inverted microscope (NEXCOPE, USA), with a clone defined as containing ≥ 50 cells. .. hPDLSCs in P3 were harvested and cultured in osteogenic differentiation medium (α-MEM supplemented with 10% FBS, 10 mmol/L β-glycerophosphate (Solarbio, China), 0.1 μmol/L dexamethasone (Solarbio, China) and 0.05 mmol/L vitamin C(Sigma; Merck, Germany) or adipogenic differentiation medium (α-MEM supplemented with10% FBS, 200 μmol/L indomethacin (Solarbio, China), 10 μmol/L dexamethasone (Sigma; Merck, Germany) and 0.5 mmol/L IBMX (Solarbio, China) for 21 days. .. Then, cells were fixed and stained with Alizarin Red S (Solarbio, China) for osteogenic differentiation or Oil Red O (Solarbio, China) for adipogenic differentiation respectively.

    Staining:

    Article Title: Polydopamine-functionalized nanohydroxyapatite coated exosomes with enhanced cytocompatibility and osteogenesis for bone regeneration
    Article Snippet: Background: Mesenchymal stem cells-derived exosomes, crucial in regenerative medicine, have been explored for their potential for the functional modification of bone scaffolds.. Objective: To design a functionally modified biomimetic nanohydroxyapatite using exosomes and explore its effects on bone regeneration.. Methods: A biomimetic nanohydroxyapatite (named as tHA) was fabricated as previous methods using a polydopamine (pDA) structure as a template, and exosomes (Exo) derived from periodontal ligament stem cells (PDLSCs) were used to functionally modify the tHA scaffold material through pDA.

    other:

    Article Title: TNF-α activates RELA expression via TNFRSF1B to upregulate OPA1 expression and inhibit chondrogenic differentiation of human adipose stem cells
    Article Snippet: Adipogenic differentiation medium , 89%H-DMEM + 1%(Streptomycin and Penicillin) + 10% FBS + 10 μg/ml Insulin + 1 μM Dexamethasone (Solarbio, China) + 200 μM Indomethacin + 0.5 mM 3-Isobutyl-1-methylxanthine(IBMX).



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    Beijing Solarbio Science adipogenic differentiation medium
    Isolation, culture, and characterization of human dental pulp stem cells (HDPSCs). (A) HDPSCs (P0) were observed using an optical microscope on day 7 (×100). (B) HDPSCs (P1) were observed using an optical microscope on day 2 (×40). (C) HDPSCs (P3) were observed using an optical microscope on day 2 (×40). (D) HDPSC phenotype was analyzed using flow cytometry. (E) <t>Adipogenic</t> differentiation of HDPSCs was evaluated using Oil red O staining at day 21 (×200). (F) Mineralized matrix formation in HDPSCs was ascertained using Alizarin red S staining at day 21 (×100).
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    Image Search Results


    Isolation, culture, and characterization of human dental pulp stem cells (HDPSCs). (A) HDPSCs (P0) were observed using an optical microscope on day 7 (×100). (B) HDPSCs (P1) were observed using an optical microscope on day 2 (×40). (C) HDPSCs (P3) were observed using an optical microscope on day 2 (×40). (D) HDPSC phenotype was analyzed using flow cytometry. (E) Adipogenic differentiation of HDPSCs was evaluated using Oil red O staining at day 21 (×200). (F) Mineralized matrix formation in HDPSCs was ascertained using Alizarin red S staining at day 21 (×100).

    Journal: Preventive Nutrition and Food Science

    Article Title: Flavonoids of Cuscuta chinensis Enhance Osteogenic Differentiation in Dental Pulp Stem Cells via the Regulation of Wnt/β-Catenin Signaling

    doi: 10.3746/pnf.2025.243

    Figure Lengend Snippet: Isolation, culture, and characterization of human dental pulp stem cells (HDPSCs). (A) HDPSCs (P0) were observed using an optical microscope on day 7 (×100). (B) HDPSCs (P1) were observed using an optical microscope on day 2 (×40). (C) HDPSCs (P3) were observed using an optical microscope on day 2 (×40). (D) HDPSC phenotype was analyzed using flow cytometry. (E) Adipogenic differentiation of HDPSCs was evaluated using Oil red O staining at day 21 (×200). (F) Mineralized matrix formation in HDPSCs was ascertained using Alizarin red S staining at day 21 (×100).

    Article Snippet: Adipogenic differentiation medium (Cat. no. PD-016) was bought from Procell; Oil red O (ORO) solution from Beijing Solarbio Science & Technology Co., Ltd., TFCC from DESITE, and the Wnt inhibitor-XAV-939 (Cat. no. HY-15147)-was from MCE.

    Techniques: Isolation, Microscopy, Flow Cytometry, Staining